Building IP: BLUE Patent Application Re "BCMA CHIMERIC ANTIGEN RECEPTORS"

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FIG. 1 shows a schematic of humanized anti-B cell maturation antigen (anti-BCMA) CAR constructs. The improved compositions and methods of adoptive cell therapy disclosed herein, provide genetically modified immune effector cells that can readily be expanded, exhibit long-term persistence in vivo, and reduce impairment of humoral immunity by targeting B cells expression B cell maturation antigen (BCMA, also known as CD269 or tumor necrosis factor receptor superfamily, member 17; TNFRSF17). As would be appreciated by those of ordinary skill in the art, a washing step may be accomplished by methods known to those in the art, such as by using a semiautomated flowthrough centrifuge. The practice of the invention will employ, unless indicated specifically to the contrary, conventional methods of chemistry, biochemistry, organic chemistry, molecular biology, microbiology, recombinant DNA techniques, genetics, immunology, and cell biology that are within the skill of the art, many of which are described below for the purpose of illustration. When the Cholera epidemic reached England, it provided another opportunity to compare homeopathic treatment with the conventional methods of the day

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Useful vectors include, for example, plasmids (e.g., DNA plasmids or RNA plasmids), transposons, adult Flash chat cosmids, bacterial artificial chromosomes, and viral vectors. The nucleic acid cassette contains the gene of interest, e.g., a CAR. 44(2):283-92, and Kozak, 1987. Nucleic Acids Res. Viral particles will typically include various viral components and sometimes also host cell components in addition to nucleic acid(s). The term "vector" is used herein to refer to a nucleic acid molecule capable transferring or transporting another nucleic acid molecule. The sense strand need only be sufficiently complementary with the antisense strand to maintain the overall double-strand character of the molecule. Moreover selfishness and acquisitiveness as human character may not be the dominant characters of human beings and inequality based on class and gender is not necessary characteristic of human society. The positive selectable marker may be a gene which, upon being introduced into the host cell expresses a dominant phenotype permitting positive selection of cells carrying the gene. Typically, the host cell comprises the viral replication transactivator protein that activates the replicat

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A constitutive expression control sequence may be a "ubiquitous" promoter, enhancer, or promoter/enhancer that allows expression in a wide variety of cell and tissue types or a "cell specific," "cell type specific," "cell lineage specific," or "tissue specific" promoter, enhancer, or promoter/enhancer that allows expression in a restricted variety of cell and tissue types, respectively. All anti-BCMA CAR T cells tested caused comparable K562-BCMA cell cytotoxicity. Illustrative examples of therapeutic antibodies suitable for combination with the CAR modified T cells contemplated herein, include but are not limited to, bavituximab, bevacizumab (avastin), bivatuzumab, blinatumomab, conatumumab, daratumumab, duligotumab, dacetuzumab, dalotuzumab, elotuzumab (HuLuc63), gemtuzumab, ibritumomab, indatuximab, inotuzumab, lorvotuzumab, lucatumumab, milatuzumab, moxetumomab, ocaratuzumab, ofatumumab, rituximab, siltuximab, teprotumumab, and ublituximab. Examples of categories of animal viruses useful as vectors include, without limitation, retrovirus (including lentivirus), adenovirus, adeno-associated virus, herpesvirus (e.g., herpes simplex virus), poxvirus, baculovirus, papillomavirus, and papovavirus (e.g., SV40). Epstein Barr virus (EBV) and Kaposi's sarcoma herpes virus (KSHV) are also examples of a gamma herpesvirus. In a particular aspect, the lymphotrophic herpes virus may be Epstein Barr virus (EBV), Kaposi's sarcoma herpes virus (KSHV), Herpes virus saimiri (HS), or https://wiki.redescomunitarias.colnodo.apc.org/index.php?title=usuario:leesa223092 Marek's disease virus (M